Source: the 1991 Census, Crown Copyright. ESRC purchase.

Both surveys preferentially sampled cattle groups composed only of store MEK162 cost (i.e. weaned cattle before finishing for slaughter) or finishing cattle closest to sale or slaughter. If such groups did not exist, one or more mixed groups with store or finishing cattle closest to sale or slaughter were sampled. From each group fresh faecal pats were sampled. The number of faecal pats tested in each group was determined from the number of cattle in the group using a prescribed sampling schedule. For the SEERAD survey, sufficient numbers of faecal pats were tested to ensure prospectively an 80% chance of sampling at least one positive pat if there was a shedding prevalence of at least 2% within the group [28]. Based on results from the SEERAD survey, in the IPRAVE survey, it was assumed that, on average, 8% of the animals in positive groups would be shedding, with shedding distributed as seen in the SEERAD survey VS-4718 mw [28]. For each IPRAVE group, sufficient fresh pat samples were taken to ensure prospectively a mean 90% probability of detecting shedding of E. coli O157 if at least one shedding animal was indeed present. Samples were collected from freshly voided faecal pats, refrigerated at 5°C as soon as possible and processed within 48 hours of collection. No direct

animal sampling was involved and the study was not regulated by The Animals ID-8 (Scientific Procedures) Act 1986. At present the SEERAD and IPRAVE data are not available on open-access databases,

however, requests for data can be made though the corresponding author. Immunomagnetic Separation (IMS) and Phage OICR-9429 mouse typing of Livestock samples Within 48 hours of sampling, one gram of faeces from each sample was tested for the presence of E. coli O157 as previously described [43]. Following IMS, one E. coli O157 isolate from each faecal sample was submitted to the Scottish E. coli O157/VTEC Reference Laboratory (SERL) for phage typing [44], and tested for the presence of genes encoding the virulence factors verocytotoxin 1 (vtx 1 ), verocytotoxin 2 (vtx 2 ) and intimin (eae) using multiplex PCR [45, 46]. Human Case Identification, Data Collection and Phage Typing Health Protection Scotland (HPS) receives reports of human cases of E. coli O157 infection from SERL and from diagnostic laboratories throughout Scotland. Diagnostic laboratories submit samples (isolates, faeces and sera) to SERL for further testing in line with Scottish guidance [47]. Using a series of phenotypic and genotypic tests, SERL confirms the identity of submitted isolates of E. coli O157, or identifies and isolates E. coli O157 from submitted faecal samples [48]. SERL also types all isolated organisms using a hierarchical array of methods including phage typing, polymerase chain reaction (PCR) and pulse-field gel electrophoresis (PFGE). The results of phage and verotoxin typing undertaken by SERL are also reported to HPS.