In this study, the authors investigated Ruxolitinib supplier the lactate and glucose dynamics during a Greco-roman wrestling match in three different weight classes. The objective of this research was to determine whether there were significant differences in the measured concentrations of lactate and glucose before, during, and after a wrestling match between lightweight, middleweight, and heavyweight youth wrestlers. Material and Methods Subjects The study was conducted with 60 youth wrestlers, 15�C20 years old, who were junior and cadet (according to international wrestling rules) members from 13 Croatian wrestling clubs. Each of the subjects participated in the Croatian Greco-Roman wrestling championship for juniors or cadets and placed between the first and tenth place.
Wrestlers that placed below the tenth position were not considered for this study because some of them were beginners and it was unclear whether we could measure the impact of wrestling training. Differences in anaerobic energy production from glycolysis occur in later years ( Korhonen et al., 2005 ). Therefore, it is reasonable to observe these age categories as a group. The sample was divided into three weight categories: lightweight (n = 20; 57 �� 6 kg), middleweight (n = 20; 70 �� 2 kg) and heavyweight (n = 20; 88 �� 13 kg). The study protocol was approved by the ethical committee of the Faculty of Kinesiology in Split (Croatia) and written informed consent to participate in the study was signed by each subject or his parents prior to commencement.
Measures Ten physiological variables for each weight category were measured: Lactate concentration before the match��after the warm-up, Lactate concentration after the first bout, Lactate concentration after the second bout, Lactate concentration after the third bout, Lactate concentration in the 5th min of recovery, Glucose concentration before the match��after the warm-up, Glucose concentration after the first bout, Glucose concentration after the second bout, Glucose concentration after the third bout, Glucose concentration in the 5th min of recovery. Procedures The concentration of lactate in blood was measured using the Accutrend lactate device; the validity was established by Baldari ( Baldari et al., 2009 ). The amount of glucose in blood was determined using an Accu-Chek Active device, and validity was established by Freckmann ( Freckmann et al.
, 2010 ). Heart rate was measured using the Polar PE3000 Heart Rate Monitor (Polar Electro Oy, Kempele, Finland). For the purpose of calculating body mass index, the subjects�� body mass and height were measured. Body mass was measured with a medical scale and a Martin��s Drug_discovery anthropometer was used for measuring body height. Subjects were instructed to follow a normal lifestyle by maintaining daily habits and avoiding any medication, alcohol, and caffeine as well as vigorous exercise within 24 hours of the test.