6%) [see Additional file 1 - Table S1]. The data was analyzed to determine if the pherotypes were randomly distributed among the R428 cost population or if there were associations with particular characteristics of the isolates, namely serotype, antibiotic resistance and the genetic lineages identified by pulsed-field gel electrophoresis (PFGE) profiling and MLST. As a first

approximation we used the Wallace coefficient (W) [26, 27]. W provides an estimate of the probability of two www.selleckchem.com/products/Adriamycin.html strains sharing the same pherotype if they share another characteristic such as serotype or being classified in the same PFGE cluster. Table 1 shows the W values obtained, indicating that isolates sharing the same serotype have a high probability of belonging to the same pherotype (W = 0.730) and this probability is higher if the isolates belong to the same PFGE cluster (W = 0.771). Both values are significantly

different from the expected values in case of a random association between pherotype and either of these two characteristics (Wi = 0.584), demonstrating that pherotypes are not randomly dispersed within the pneumococcal population. Table 1 Wallace’s coefficients and respective confidence intervals testing the ability of several methods to predict the pherotype. Parameter W (95% CI) Wi a Serotype 0.730 (0.689;0.772) 0.584 PFGE cluster 0.771 (0.726;0.816) 0.584 Sequence type 0.982 (0.964;1) 0.621 PI3K Inhibitor Library cell line Clonal complex 0.986 (0.961;0.992) 0.621 aWi is the expected Wallace coefficient if the classification method is independent of the pherotype. To determine if individual serotypes Tolmetin and PFGE clusters were significantly enriched in isolates presenting each pherotype, odds ratios (OR) were calculated. A total of five serotypes are significantly associated with either one of the pherotypes (Table 2 and see Additional file 1 – Table S1). The high Wallace values suggest that pherotype/serotype association is not only due to these

five serotypes. Many serotypes are present in insufficient numbers to reach a significant odds ratio. By simultaneously looking at each pair of strains the Wallace statistic has an increased power to detect associations. Serotypes 1 and 14 are strongly associated with CSP-1 whereas serotypes 3, 6A and 9N show an association with CSP-2. The same approach was used to determine if pherotypes were associated with particular PFGE clusters within each serotype, aiming to subdivide serotypes into closely related genetic lineages. Five PFGE clusters showed association with a particular pherotype [see Additional file 2 - Table S2]. Of these, the largest PFGE clusters within serotypes 1, 3, 9N and 14 maintained the same association found between these serotypes and pherotype.