We reasoned that B 1,three glucanase hydroly ses B 1,3 glucans not merely from the fungal cell wall, but also flax callose, an endogenic B one,3 glucan. Because the level of callose was decreased during the fibres from transgenic line B14 relative towards the ranges for your management fibres, we speculate the excess of glucose units may be used as substrate for that synthesis of other wall polymers. The thorough analysis in the wall constituents uncovered significant distinctions in the articles of phenolic compounds bound using the pec tin and hemicellulose fractions. 3 phenolic acids, two phenolic alde hydes and one flavonoid had been identified in the pectin fractions isolated from fibres. The total content of phenolic compounds from the fibres through the transgenic plants was about 26% increased than during the fibres through the manage plants.
On the wall fractions that had been analysed, CSF selleck chemicals and K4SF were characterised by a significant boost from the contents of almost all of the phenolic compounds. In K4SF, this is often prob ably induced by the enhance in xylose material in the transgenic flax fibres, since it was reported that phenolic acids bind preferentially to arabinoxylans. From the remaining fractions, the quantities of particular com lbs have been different and depended over the fraction along with the compound analysed. The obtained success had been reflected inside the antioxidant properties of individual fractions. The highest antioxi dant potential was shown through the K1SF from the fibres through the transgenic flax. It’s well worth noting that regardless with the very similar excellent of phenolic compounds in the WSF, NSF and K4SF, the hemicellulose fraction displayed the lowest skill to inhibit the reactive form DPPH.
It is actually speculated that besides the phenolic compound contents, selleck inhibitor the proper composition and structure of pectin might also influence the antioxidant properties. It is interesting to note that the antioxidant action of every one of the wall fractions from flax fibres was statistically larger than that for com mercially accessible pectin. The results confirmed that flax fibre pectin is probably more helpful than industrial citrus pectin and indicated the chance of its biomedical application. Conclusion Fibres from flax plants overexpressing the B one,three glucanase gene have an altered wall biopolymer composition. The contents of individual polysaccharides have been increased, but lowered lignin information was also observed. A lessen within the information of callose, that’s a substrate for B 1,three glucanase, was also uncovered. It can be therefore advised that the glucose units released from the callose are redirected to your production of other polysac charides. The increase during the contents of monosaccharides, the main component of hemicellulose, is accompanied by an accumulation of phenolic compounds on this fraction.