The relatively fast computing time was a result of reducing the objective function to 10-4 and limiting the data points to three decimal

places in the input dataset. The objective function is the summed squared mean distance measured between the simulated data and input data. Reducing the objective function increased computing time but improves the quality of the parameter fit to input data. We performed an experiment to determine the relationship between the value of the objective function and the time taken to compute PVA for one reaction with two substrates, two products, one enzyme and six kinetic Inhibitors,research,lifescience,medical parameters (Figure 5). The results of this experiment Alpelisib cell line indicate that the computing time for parameter

estimation increases significantly when the objective function is reduced to 10-10 and beyond. The relationship that is observed between the objective function and computing time appears to be linear (PVA was computed on a desktop computer with a quad CPU having 3.00 GHz, 2.99 GHz processor speed Inhibitors,research,lifescience,medical and 4 GB of RAM). Figure 5 Computing times of parameter variability analysis (PVA) against changes in objective function. PVA was performed for a reaction with two substrates, two products, one enzyme and six kinetic parameters. For each PVA run, Inhibitors,research,lifescience,medical the summed squared mean distance … Another variable that can increase computing time in parameter estimation is the number of data points in the experimental dataset. To examine how

the number of data points influences computing time, we performed parameter estimation for a single reaction with two substrates, two products, one enzyme Inhibitors,research,lifescience,medical and six kinetic parameters (Figure 6). The result of this experiment indicates that the Inhibitors,research,lifescience,medical number of data points in the input dataset for parameter estimation increases the computing time in a non-linear manner. This explains why a relatively fast time of 5 hours and 40 minutes was recorded when PVA was performed for such a large model with 2537 kinetic parameters as the number of input data points was only three. Figure 6 Relationship between number of Levetiracetam input data points and computing time. PVA was performed for a single reaction of two substrates, two products, one enzyme and six kinetic parameters. PVA was repeated six times and for each iteration the number of data points … 3.5. Validation on Model Integrity We tested the predictive capability of our M. tuberculosis model and kinetic parameters by determining whether different conditions could be predicted without re-estimating kinetic parameters. To calibrate the model over a range of glycerol uptakes, we created a virtual time series containing ten repeats of each of the steady-state flux distributions obtained from input conditions with glycerol at 0, 0.5 and 1 mmol/gDW/h, respectively.