Peptide mass fingerprints identified in the affinity-purified material were used to identify L. interrogans proteins by searching against the NCBInr bacterial genome database. Acknowledgments We thank Ajit Varki and Victor Nizet for valuable advice and Sandra Diaz for technical assistance with HPLC-MS. The Scripps Research Institute’s Center for Mass Spectrometry performed nano-flow MS/MS data and provided results of the NCBInr database search (http://​masspec.​scripps.​edu/​). This work was supported in part by U.S. Public Health Service grants from the National Institutes of Health 1D43TW007120 and 1RO1TW05860. References 1. Bharti AR, Nally

JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, et al.: Leptospirosis: a zoonotic Selleckchem CHIR-99021 disease of global importance. {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| Lancet Infect Dis 2003,3(12):757–771.PubMedCrossRef 2. McBride AJ, Athanazio DA, Reis MG, Ko AI: Leptospirosis. Curr Opin Infect Dis 2005,18(5):376–386.PubMedCrossRef 3. Ristow P, Bourhy P, da Cruz McBride FW, Figueira CP, Huerre M, Ave P, Girons IS, Ko AI, Picardeau M: The OmpA-like protein Loa22 is essential for leptospiral virulence. PLoS Pathogens

2007,3(7):e97.PubMedCrossRef 4. Wu BT, Bao L, Sun Z, Li DK, Zhang Y: [The OmpA-like protein Loa22 from Leptospira interrogans check details serovar lai induces apoptosis in A549 via Ca2+ signal pathway]. Journal of Sichuan University Medical Science Edition 2011,42(3):298–302.PubMed 5. Zhang Y, Bao L, Zhu H, Huang B, Zhang H: OmpA-like protein Loa22 from Leptospira interrogans serovar Lai is cytotoxic to cultured rat renal cells and promotes inflammatory responses. Acta Biochimica et Biophysica Sinica 2010,42(1):70–79.PubMedCrossRef 6. Hoke DE, Egan S, Cullen PA, Adler B: LipL32 is an extracellular matrix-interacting protein of Leptospira spp. and Pseudoalteromonas tunicata. Infect Immun 2008,76(5):2063–2069.PubMedCrossRef 7. Murray GL, Srikram A, Hoke DE, Wunder EA, Henry R, Lo M, Zhang K, Sermswan Fossariinae RW, Ko AI, Adler B: Major surface protein

LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infect Immun 2009,77(3):952–958.PubMedCrossRef 8. Stevenson B, Choy HA, Pinne M, Rotondi ML, Miller MC, Demoll E, Kraiczy P, Cooley AE, Creamer TP, Suchard MA, et al.: Leptospira interrogans endostatin-like outer membrane proteins bind host fibronectin, laminin and regulators of complement. PLoS One 2007,2(11):e1188.PubMedCrossRef 9. Barbosa AS, Abreu PA, Neves FO, Atzingen MV, Watanabe MM, Vieira ML, Morais ZM, Vasconcellos SA, Nascimento AL: A newly identified leptospiral adhesin mediates attachment to laminin. Infect Immun 2006,74(11):6356–6364.PubMedCrossRef 10. Yuri K, Takamoto Y, Okada M, Hiramune T, Kikuchi N, Yanagawa R: Chemotaxis of leptospires to hemoglobin in relation to virulence.