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Bioinformatics 2011, 27:2194-2200.PubMedCrossRef 51. Wang Q, Garrity GM, Tiedje JM, Cole JR: Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 2007, 73:5261-5267.PubMedCrossRef 52. White JR, Arze C, Team TC, Matalka M, White O: CloVR-16S: Phylogenetic microbial community composition analysis based on 16S ribosomal RNA amplicon sequencing – standard operating procedure, version 1.1. http://precedings.nature.com/documents/5888/version/2 Temsirolimus cell line Authors’ contributions JK, AO, and TL conceived the study and participated in its design. AO and TL performed all lab work. JW performed data analysis. TL drafted the manuscript. AO, JW, JK, MA, and EB contributed to the draft of the manuscript. All authors read and approved the final manuscript.”
“Background Streptomyces species are widely distributed in natural habitats, such
as soils, lakes, plants and some extreme environments [1, 2]. They are Gram-positive, mycelial bacteria with high G+C content (often >70%) in their DNA [3]. More selleck products than 6000 antibiotics and pharmacologically active metabolites (e.g. antiparasitic and antitumor agents, immuno-suppressants etc.) have been discovered in Streptomyces species [4]. Streptomyces species usually harbor conjugative plasmids [5]. Modes of plasmid replication in Streptomyces include rolling-circle (RC) (e.g. pIJ101, pJV1, pSG5, pSN22, pSVH1, pSB24.2, pSY10 and pSNA1) [6], and uni-directional or bi-directional theta types (e.g. SCP2, pFP11 and pFP1) [7, 8]. Some plasmids (e.g. SLP1 and pSAM2) replicate in chromosomally-integrating/autonomous forms [9–11]. Streptomyces RC plasmids are usually small (8–13 kb), while theta-type plasmids are larger (31–120 kb).