Having said that, based on their similar mass spectra, we tenta tively presume that they are structurally relevant, Beside the title crematoenone for com pound 10 we hence propose to make use of the plural kind crematoenones for that entire substance class, reflecting the single double bond within the octahydronaphtalene subunit as well as keto moiety. For two further compounds, we obtained adequate substance to carry out HRMS analysis, and according to the reconstruction and extrapolation of EI fragmentation mechanisms, we are able to propose sound chemical structures for these compounds, Compound 6 was tentatively identified as 2,three dihydrocrematoenone. In compound 18, the double bond of your alkenyl moiety was almost certainly replaced by an O acetyl performance, i. e. compound 18 would be an O acetyldihydrocrematoenone. Behavioural assays.
Camponotus in direction of Crematogaster The behavioural assays were to find out the part of hy drocarbons vs. crematoenones in nestmate recognition. We confronted a Camponotus rufifemur colony with dead Crematogaster modiglianii workers from its partner colony and from a non nestmate Cr. modiglianii colony, and measured read the article regardless of whether the Ca. rufifemur aggression differed be tween intracolonial and allocolonial Cr. modiglianii. In three subsequent treatment options, we similarly established whether or not Ca. rufifemur distinguished their partner in the non nestmate colony, however the Ca. rufifemur ants have been only confronted with cues, presented on odourless dummies, These cues have been complete cu ticular extracts, cuticular hydrocarbons only, and crematoenones only, each time from intra and allocolonial Cr. modiglianii.
The cuticular hydrocarbons of different Cr. modiglianii Telaprevir colonies show only quantitative variations. on the other hand, individuals colonies living together with the red Ca. rufifemur variety possess two hydrocarbons that are absent from those residing with the black variety, Camponotus rufifemur did not differentiate concerning dead intracolonial and dead allocolonial Cr. modiglianii staff. Nevertheless, they discriminated between their extracts and especially concerning their hydrocarbon frac tions. Cuticular extracts of allocolonial Cr. modiglianii elicited substantially more aggression than intracolonial ones. The hydrocarbon fractions of allocolonial Cr. modiglianii triggered quite large aggression, whilst hydrocarbon fractions of intracolonial Cr. modiglianii have been handled amicably, This differential aggression was extremely signifi cant.
In contrast, the behaviour towards the crematoenone fractions was primarily peaceful for each intracolonial and allocolonial cues. Similarly, a re mixture of hydrocarbon and crematoenone fractions of allocolonial Cr. modiglianii received little aggression, which corresponds to your weaker differentiation concerning the two complete extracts in contrast towards the two hydrocarbon fractions.