None of the SSc sSS patients had active lung inter stitial disease or pulmonary hypertension and none was using immunosuppressive drugs when the salivary sam ples were collected. sellckchem In the second part of the Inhibitors,Modulators,Libraries study, the independent set cohort included 75 unselected, consecutive Inhibitors,Modulators,Libraries subjects, 21 healthy volunteers, 19 patients with a diagnosis of pSS, 10 patients with non SS sicca syn drome and 25 sec ondary SS and 17 SSc sSS. Demographic and glandular manifestations of the valida tion cohort did not differ from the derivation cohort of the subjects included in the first phase of the study. Saliva collection and processing Between 1 and 3 ml of saliva were obtained from each subject. No differences were detected in the mean volume of saliva collected from each group of patients.

In particular, we obtained a mean volume of 1. 05 0. 77 ml of saliva from patients with pSS, 1. 02 0. 78 ml from patients with non SS sicca symptoms and 1. 06 0. 49 ml and 1. 07 0. 34 from patients with RA sSS and SSc sSS respectively. Identification of differentially expressed salivary proteins in pSS with respect to healthy, RA sSS, SSc sSS Inhibitors,Modulators,Libraries and non SS sicca syndrome Typical 2DE gel images of exemplary gels of salivary protein extracts from pooled samples of healthy volun teers, pSS patients, RA sSS, SSc sSS and non SS sicca syndrome subjects are shown in Figures 1 and 2. By computational gel image comparison, a total of 28, 6, 7 and 12 protein spots were found to be differentially expressed in healthy volunteers, non SS sicca syndrome, SSc sSS and RA sSS salivary profiles, with respect to pSS samples.

For representative salivary proteins, the identification was obtained by direct comparison with images of previous studies. For unknown proteins, we proceeded with MS MS analysis. Of Inhibitors,Modulators,Libraries the 15 cut spots, 80% were identified by MS MS spectrometry, yielding 9 different Inhibitors,Modulators,Libraries protein identifications. A list of identified pro teins, MW, isoelectric point score and coverage values of MS MS is shown in Table 2. Table 3 summarises the most significant differences among pSS patients, healthy volunteers and non SS sicca syndrome in the expression of both representa tive and newly identified salivary proteins. In addition, the fold changes of the expression of previously and newly identified proteins in RA sSS and SSc sSS subjects with respect to pSS, and the relative P values, are shown in Table 4.

The proteins found to be differentially expressed are also circled in the representative gels. As selleck chem inhibitor described in Table 3, 12 proteins were differently expressed in pSS with respect to healthy volunteers. Among them, six proteins showed a 1. 5 fold of increase in pSS, psoriasin, IGKC protein and six were characterised by a 1. 5 fold decrease, short palate, lung and nasal epithelium clone 2, glyceraldehydes 3 phosphate dehydrogenase.