Melondialdehyde formed is reacted with thiobarbituric acid and a colored florescent product is formed. Percentage radical scavenging was calculated using the following formula: %Inhibition=[(Acontrol−(Asample−Asampleblank)/Acontrol]×100 The scavenging activity of the different extracts toward superoxide anion radicals was measured by the method

of Nishimiki14 with slight modifications. The superoxide radical generated from dissolved oxygen by PMS–NADH check details coupling measured by their ability to reduce NBT. The decrease in absorbance at 562 nm with the plant extracts indicated their ability to quench superoxide radicals in the reaction mixture. The % inhibition of superoxide anion generation was calculated using the following formula: %Scavenging=[(Acontrol−(Asample−Asampleblank)/Acontrol]×100 In this present study the antioxidant activity of various extracts of Mentha species have been investigated. Initial studies revealed only aqueous and methanolic extracts exhibited reasonable antioxidant activity, so the work was carried out with these solvents. CHIR-99021 research buy These extracts were assayed for their total phenolic and flavonoid content and antioxidant activities by using different in vitro models. It is evident from the results (Table 1) that the leaves of M. spicata had a higher content

of total phenols and flavanoids in plants raised at either of the altitudes as compared to M. longifolia. The results also revealed that the total phenolic and flavonoid content of both the species was higher in second generation leaves as compared to the respective first generation leaves of plants raised at either of the locations. Moreover the total phenolics and flavonoid content of both the species of Mentha raised at K.U Srinagar was much higher than the corresponding species raised at L.P.U Phagwara. Fe (III) reduction is often used as an indicator of electron donating activity, which is an important mechanism of phenolic antioxidant action.15 Reducing power is associated with its antioxidant activity and may serve very as a significant reflection of the antioxidant activity.16 Compounds with reducing power indicate that they are electron donors and

can reduce the oxidized intermediates of lipid peroxidation processes, so that they can act as primary and secondary antioxidants.10 and 17 Their studies have indicated that the antioxidant effect is related to the presence of reductones.10 Reductones are reported to be terminators of free radical chain reactions,18 thus, the antioxidant activity of extracts observed may be related to its reductive activity. Total reducing power of different solvent extract is shown in Table 2. The results that the total reducing power of M. spicata was substantially higher in both the extracts at both the altitudes as compared to M. longifolia. The results also revealed that the total reducing power of first generation leaves of both the species was much higher than second generation leaves except M.