Differentiation of fibroblasts to myofibroblasts was induced in the in vivo wound-healing model and was confirmed in vitro using the rat fibroblastic cell line Rat-1. Cyclooxygenase (Cox)-2 expression was also induced in Rat-1 cells by 3-oxo-C12-HSL. This finding suggested that Cox-2 upregulation may be related to the inflammatory findings in the histological examinations, in which infiltrating polymorphonuclear neutrophils were observed selleck kinase inhibitor at the wound site. Taken together, these results imply that mammals have a potential defense system against invading pathogens by responding to the presence of 3-oxo-C12-HSL and inducing the differentiation
of fibroblasts to myofibroblasts as well as inflammation for accelerating wound healing.”
“Purpose: To assess the feasibility and effectiveness of rapid right ventricular selleck pacing with a magnetic resonance (MR)-compatible pacemaker lead during MR-guided aortic valvuloplasty.
Materials and Methods: This study was approved by the institutional animal research committee. Seven pigs were investigated. All experiments were performed with an interventional 1.5-T MR system. Interventions were monitored with a steady-state free precession real-time imaging sequence. An MR-compatible pacemaker lead was placed in the right ventricular apex with MR guidance before valvuloplasty. After positioning the balloon in valve position, valvuloplasty was performed with rapid right
ventricular rapid pacing at a heart rate of 180 beats per minute to minimize cardiac output.
Results: Positioning of the pacemaker lead with MR guidance was feasible in all swine (sensing, 6 mV +/- 1; threshold, 1 V +/- 0.5). The lead could be seen on steady-state
free precession images without inducing any artifacts. Rapid right ventricular pacing was feasible in all swine, and balloon stability Selleckchem JNK-IN-8 at the time of inflation was achieved with no balloon movement. Aortic valvuloplasty was successfully accomplished in all experiments.
Conclusion: Rapid right ventricular pacing with an MR-compatible pacemaker lead is feasible and effective.”
“Pulsed systems are extensively used to produce active species such as atoms, radicals, excited states, etc. The tunable diode laser absorption spectroscopy (TD-LAS) is successfully used to quantify the density of absorbing species, but especially for stationary or slow changing systems. The time resolved-direct absorption profile (TR-DAP) measurement method by TD-LAS, with time resolution of mu s is proposed here as an extension of the regular use of diode laser absorption spectroscopy. The spectral narrowness of laser diodes, especially in the blue range (similar to 0.01 pm), combined with the nanosecond fast trigger of the magnetron pulsed plasma and long trace recording on the oscilloscope (period of second scale) permit the detection of the sputtered titanium metal evolution in the afterglow (similar to ms).