(C) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Heterozygous mutations of the tissue-specific transcription factor Transmembrane Transproters modulator hepatocyte nuclear factor (HNF)1 beta, cause maturity onset diabetes of the young (MODY5) and kidney anomalies including agenesis, hypoplasia, dysplasia and cysts. Because of these renal anomalies, HNF1 beta is classified as a CAKUT (congenital anomalies of the kidney and urinary tract) gene. We searched for human fetal kidney proteins interacting with the N-terminal region of HNF1 beta using a bacterial two-hybrid system and identified five novel proteins along with the known partner DCoH. The interactions were confirmed for
four of these proteins by GST pull-down assays. Overexpression of two proteins, E4F1 and ZFP36L1, in Xenopus embryos interfered with pronephros formation. Further, in situ hybridization showed overlapping expression of HNF1 beta, E4F1 and ZFP36L1 in the developing pronephros. HNF1 beta is present largely in the nucleus where it colocalized with E4F1. However, ZFP36L1 was located predominantly in the cytoplasm. A nuclear function for ZFP36L1 was shown as it was able to reduce HNF1 beta transactivation in a selleck inhibitor luciferase reporter system. Our studies show novel proteins may cooperate with HNF1 beta in human metanephric
development and propose that E4F1 and ZFP36L1 are CAKUT genes. We searched for mutations in the open reading frame of the ZFP36L1 gene in 58 patients with renal anomalies but found none.”
“Lens epithelium-derived growth factor (LEDGF) can be alternatively spliced to produce two isoforms-LEDGFp52 and LEDGFp75, however, LEDGFp52 has rarely been investigated. The LEDGFp52 protein or its monoclonal antibody was added to primary rat retinal ganglion cell cultures and their impact on neurite number and length, and the mRNA and protein expression levels of GAP-43, NF-L and MAP2 quantified. LEDGFp52
was compared to the addition of ciliary neurotrophic factor (CNTF). LEDGFp52 protein significantly increased primary neurite growth compared to control conditions. www.selleck.cn/products/lgx818.html In addition, the expression of GAP-43, NF-L and MAP2 genes and proteins were also significantly up-regulated. The positive action of the LEDGFp52 protein was similar to or more efficacious than CNTF. LEDGFp52 appears to be an important regulatory protein for the growth of cell processes. (C) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Renal amyloid deposits can often be seen in primary amyloidosis (immunoglobulin light chain disease) or in secondary forms such as reactive amyloidosis as well as in several hereditary forms where a variety of mutant proteins ‘precipitate’ as amyloid plaques. However, in rare cases, amyloidosis may be identified by renal biopsy, but no definitive diagnosis could be made.