BMC Biotechnol 2007, 7:34.PubMedCrossRef 63. Jensen PR, Hammer K: The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl Environ Microbiol 1998, 64:82–87.PubMed 64. Jeong H, Barbe

V, Lee CH, Vallenet D, Yu DS, Choi SH, Couloux A, Lee SW, Yoon SH, Cattolico L, Hur CG, Park HS, Ségurens B, Kim SC, Oh TK, Lenski RE, Studier FW, Daegelen P, Kim JF: Genome sequences of Escherichia coli B strains REL606 and BL21(DE3). J Mol Biol 2009,394(4):644–652.PubMedCrossRef selleck kinase inhibitor 65. Studier FW, Daegelen P, Lenski RE, Maslov S, Kim JF: Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes. J Mol Biol 2009,394(4):653–680.PubMedCrossRef 66. Chen D, Texada D: Low-usage codons and rare codons of Escherichia coli . Gene Therapy and Molecular Biology 2006, 10A:1–12. 67. Bailly-Bechet M, Danchin A, Iqbal M, Marsili M, Vergassola M: Codon usage domains MDV3100 order over bacterial chromosomes. PLoS Comput Biol 2006,2(4):e37.PubMedCrossRef 68. Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 2000, 97:6640–6645.PubMedCrossRef 69. Waegeman H, Beauprez J, Maertens J, Mey MD, Demolder L, Foulquié-Moreno MR, Boon N, Charlier D, Soetaert W: Validation study of 24 deepwell microtiterplates to screen libraries of strains in metabolic engineering. J Biosci Bioeng 2010,110(6):646–652.PubMedCrossRef

70. Fischer E, Zamboni N, Sauer U: High-throughput metabolic flux analysis based on gas CB-839 solubility dmso chromatography-mass spectrometry derived 13 C constraints. Anal Biochem 2004, 325:308–316.PubMedCrossRef 71. Duetz W, Witholt B: Oxygen transfer by orbital shaking of square vessels and deepwell microtiter plates of various dimensions. Biochem Eng J 2004, 17:181–185.CrossRef 72. Nanchen A, Schicker A, Sauer U: Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coli . Appl Environ Microbiol Abiraterone clinical trial 2006,72(2):1164–1172.PubMedCrossRef 73. Notley-McRobb L, Death A, Ferenci T: The relationship between external glucose concentration and cAMP levels inside

Escherichia coli : implications for models of phosphotransferase-mediated regulation of adenylate cyclase. Microbiology 1997,143(Pt 6):1909–1918.PubMedCrossRef 74. Kayser A, Weber J, Hecht V, Rinas U: Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. I. Growth-rate-dependent metabolic efficiency at steady state. Microbiology 2005,151(Pt 3):693–706.PubMedCrossRef 75. Parrou JL, Francoois J: A simplified procedure for a rapid and reliable assay of both glycogen and trehalose in whole yeast cells. Anal Biochem 1997, 248:186–188.PubMedCrossRef 76. Maloy SR, Bohlander M, Nunn WD: Elevated levels of glyoxylate shunt enzymes in Escherichia coli strains constitutive for fatty acid degradation. J Bacteriol 1980,143(2):720–725.PubMed 77.