5; p=0.03). Patients with free copper lower than 15 mcg/dL had higher HDL and lower LDL and total cholesterol levels, with statistical significance only on LDL (50±15 vs 43±11 mg/dL, p=0.1; 106±35 vs 131±36 mg/dL, p=0.01;

186±40 vs 208±44 mg/dL, p=0.08, respectively). This group also showed lower AST levels (37.7±27 vs 45.9±24 IU/L; p=0.04). Age, gender, hypertension and diabetes were also evaluated but had no statistical difference. CONCLUSIONS: Patients with NAFLD had different clinical and biochemical markers according to the levels of free copper and ceruloplasmin suggesting that alterations in the metabolism of copper DMXAA chemical structure could have some role in NAFLD development. Disclosures: The following Selumetinib molecular weight people have nothing to disclose: Vinicius Nunes, Adriana R. Andrade, Ana Luiza V. Guedes, Claudia P.

Oliveira, Marcio A. Diniz, Jose Estefano, Daniel F. Mazo, Eduardo Luiz R. Cancado BACKGROUND: Fatigue is the most common symptom in subjects with nonalcoholic fatty liver disease (NAFLD). Chronic fatigue has been associated with elevated systemic levels of pro-inflammatory cytokines. While several drugs improve liver histology in those with NAFLD, they have not been shown to impact patient symptoms. Tai chi is a complementary and alternative medicine approach to improving perceived daily stress and has been shown to improve chronic fatigue; its utility in NAFLD is unknown. AIMS: To evaluate the effects of tai chi on fatigue, depression and overall sense of wellness as well as markers of systemic inflammation and liver injury in a “proof of concept” study in subjects with NAFLD (NCT# 01467544). METHODS:A randomized controlled trial of tai chi was performed in obese non-diabetic women who met non-invasive criteria for NAFLD (AST or ALT > 30 IU/L and high liver fat scores) and no known chronic liver disease. Subjects were randomized

to either tai chi or no intervention for 8 weeks. Subjects maintained their usual diet. Fatigue was assessed by brief fatigue inventory and multi-dimensional fatigue symptoms inventory. Overall patient centered outcomes were assessed Tacrolimus (FK506) from patient-reported outcome surveys. The levels of IL-1, IL-8, IFN-γ, TNF-α were also measured along with liver enzymes as markers of liver injury. RESULTS: A total of 56 subjects were randomized to tai chi or no interventions. The two groups were well-matched with respect to age, body mass index, race, ALT, severity of insulin resistance, cytokine levels and baseline symptom scores. At the end of study, subjects in the tai chi group had improvement in all symptom scales from baseline: multidimensional fatigue inventory (16.9 to 1.6, p< 0.001), Patient-Reported Outcomes Measurement System (19.3 to 15.6, p=.01), brief fatigue inventory (4.3 to 2.9, p=.09) and Depression scores (0.6 to 0.3, p=.01). ALT also improved (24 IU/L to 21 IU/L, p=.05).