5 °C every 5 s

while monitoring the fluorescence. These assays were performed in triplicate for each strain. Student’s t test was used for statistical analysis. Acknowledgements The work in the AGT laboratory was supported by UTMB discretionary funds and partially by NIH/NIAID grant 5U01AI082103. The authors would like to thank Dr. Douglas Botkin for technical advice and support. We are grateful to Mardelle Susman for many helpful editorial suggestions on this manuscript. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIAID or NIH. Electronic supplementary material Additional file 1: Figure S1. Growth curves of E. coli O104:H4 isogenic strains. Growth curve of wild-type E. coli O104:H4 strain C3493 and its isogenic mutant CSS001 (ΔiutA) in LB or LB supplemented with 2,2’-dipyridyl (LB + DP) at Y-27632 supplier 37 °C and represented as A. CFU/mL and B. OD600. (TIFF 638 KB) Additional file 2: Figure S2. MALDI-TOF identified peptides matching the aerobactin receptor. Peptides were identified by MALDI-TOF and subjected to BLAST search analysis which resulted in identification of the Ferric aerobactin receptor precursor from Escherichia coli (gi|218692454) with a score 0f 158 and an expected value of 1.5e-11. The

sequence learn more coverage was 18% and the matched peptides are depicted as bold letters. (TIFF 615 KB) References 1. Farfan MJ, Torres AG: Molecular mechanisms mediating colonization of Shiga toxin-producing Escherichia coli strains. Infect Immun 2011, 80:903–913.PubMedCrossRef 2. Nataro JP, Kaper JB: Diarrheogenic Escherichia coli. Clin Microbiol Rev 1998, 11:142–210.PubMed 3. Frank C, Werber D, Cramer JP, Askar M, Faber M, ander Heiden M, Bernard

H, Fruth A, Prager R, Spode A, et al.: Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in ID-8 Germany. N Engl J Med 2011, 365:1771–1780.PubMedCrossRef 4. Askar M, Faber MS, Frank C, Bernard H, Gilsdorf A, Fruth A, Prager R, Hohle M, Suess T, Wadl M, et al.: Update on the ongoing outbreak of haemolytic uraemic syndrome due to Shiga toxin-producing Escherichia coli (STEC) serotype O104, Germany, May 2011. Euro Surveill 2011,16(pii):19883.PubMed 5. Scheutz F, Nielsen EM, Frimodt-Møller J, Boisen N, Morabito S, Tozzoli R, Nataro JP, Caprioli A: Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011. Euro Surveill 2011,16(pii):19889.PubMed 6. Brzuszkiewicz E, Thürmer A, Schuldes J, Leimbach A, Liesegang H, Meyer FD, Boelter J, Petersen H, Gottschalk G, Daniel R: Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC). Arch Microbiol 2011, 193:883–891.