47%; cases with history of alcohol accounted for 14.99%; HBV infection accounted for 89.74%, HBV infection duration ≥10 years accounted for 86.82%, HBV DNA ≥ 500 IU/ml accounted for 76.70%, HCV infection accounted for 9.57%, patients with cirrhosis accounted for 95.42%.there are 82.66% patients without the experience of interferon / nucleoside drug treatment. 2, After multidisciplinary intervention, primary liver cancer mortality fell from 49.73% in 2010 to 29.09% in 2012(p < 0.05), primary liver cancer mortality rate accounted for the total hospital mortality rate was 59.47% in 2010, the corresponding index was 43.94% in 2012 (p < 0.05); median survival is 9.8 ± 4.1 months and 15.23 ± 3.1 months before

and after multidisciplinary intervention respectively(p < 0.05).3, OR value that HBVDNA ≥ 500 IU/ml on primary liver cancer died within 2 years is 4.07,95% CI is 2.43 INCB024360 supplier ~ 6.75, p < 0.05;

OR value that AFP ≥ 350 ng/ml on primary liver cancer died within 2 years is 6.20, 95% CI is 3.62 ~ 10.62, p < 0.01. Trametinib cost Conclusion: 1, male, age greater than 40 years, family history, HBV infection, HBV DNA high load, duration of infection, without antiviral treatment experience, and cirrhosis are high risk factors of primary liver cancer occurring 2, Tumor multidisciplinary intervention can extend the survival of patients. HBV DNA high load and AFP high level are risk factors on primary liver cancer died within 2 years. Key Word(s): 1. HCC; 2. epidemiology; 3. risk factors; Presenting Author: FANPU JI Additional Authors: BAOHUA LI, NA HUANG, HAIYAN CHEN, JUN LI, CHANYUAN WANG, ZHIDONG WANG, KE LI, ZONGFANG LI Corresponding Author: ZONGFANG LI Affiliations: National & Local Joint Engineering Research Center of 上海皓元医药股份有限公司 Biodiagnosis and Biotherapy, The Second Affiliated Hospital, College of Medicine, Xi’an Jiaotong

University; Department of Infectious Disease, The Second Affiliated Hospital, College of Medicine, Xi’an Jiaotong University; Department of General Surgery, The Second Affiliated Hospital, College of Medicine, Xi’an Jiaotong University Objective: spleen has biphasic and bidirectional characteristics in tumor immunology. To the date, the detailed description of cellular immune status in spleen during tumor progression has not been fully investigated. In the present study, we examined the percentage of myeloid derived suppressor cell (MDSC), CD4+ T cell, CD8+ T cell, NK, NKT and macrophage (MΦ) in spleen of murine H22 transplantable hepatoma. Methods: H22 hepatoma cells (2 × 105, 20 μl) were injected into the livers of BALB/c mice. One week, two weeks and three weeks later, splenocyte suspension was prepared and stained using the following fluorescent antibody against CD11b and Gr-1 (MDSC), CD3, CD4 and CD8a (T cells), CD49b (NK, NKT), F4/80 (MΦ), and detected by flow cytometry. Results: The mean survival time of tumor-bearing mice was 21 days. In 2nd and 3rd week of tumor progression, the percentage of MDSC was markedly elevated (9.73 ± 2.31%, 22.52 ± 0.