3 7 46.7   T3 88 60 68.2 28 31.8   48 54.5 40 45.5   T4 11 9 81.8 Selleck GDC0068 2 18.2   5 45.5 6 54.5   Distant metastasis           0.504         0.797 M0 102 71 69.6 31 30.4   55 53.9 47 46.1   M1 12 10 83.3 2 16.7   6 50.0 6 50.0   TNM staging           0.431         0.297 I 11 9 81.8 2 22.2   5 45.5 6 54.5   II 47 30 63.8 17 36.2   21 44.7 26 55.3   III 44 32 72.7 12 27.3   28 63.6 16 36.4   IV 12 10 83.3 2 16.7   7 58.3 5 41.7   a median, 59 years; b mean,

5.0 cm; c R/DM-Recurrence/distant metastasis; d lymphocytic infiltration in the tumor interstitial VEGF expression was statistically significant difference with lymph node metastasis, and was significantly correlated with TNM staging (P < 0.05, r = 0.302) (Table 3). Table 3 Relationship of VEGF expression and MVD with clinicopathologic parameters and SPARC expression Parameters   VEGF P value MVD (CD34) P value     (-) (1+) (2+) (3+)   (mean ± S.D.) (ANOVA) Total 114 31 27 22 34   11.60 ± 5.68   Age           0.612   0.319 PI3K inhibitor < 59 48 11 10 10 17   12.23 ± 6.19   ≥ 59 66

20 17 12 17   11.15 ± 5.28   Tumor differentiation           0.112   0.952 low 16 6 2 3 5   11.24 ± 7.30   moderate 68 16 18 9 25   11.72 ± 5.30   high 30 9 7 10 4   11.53 ± 5.75   Lymph node metastasis           0.001   0.879 N0 65 23 20 13 9   11.80 ± 5.54   N1 36 7 6 7 16   11.20 ± 6.74   N2 13 1 1 2 9   11.74 ± 2.59   depth of invasion           0.601   0.281 T2 15 5 3 4 3   11.28 ± 5.63   T3 88 24 21 14 29   11.33 ± 5.66   T4 11 2 3 4 2   14.20 ± 5.72   TNM staging           0.002   0.295 I 11 4 3 3 1   12.00 ± 6.00   II 47 17 15 8 7   10.99 ± 4.70   III 44 8 6 6 24   11.04 ± 6.26   IV 12 2 3 5 2   14.26 ± 5.46   SPARC in MSC           0.0001   0.027 low Oxymatrine reactivity 61 17 6 13 25   12.69 ± 5.71   high reactivity 53 14 21 9 9   10.34 ± 5.43   Correlation analysis of SPARC expression

in MSC with VEGF expression and MVD Using Spearman rank correlation analysis, SPARC expression in MSC was negative significantly related with VEGF in colon cancer tissue (P < 0.05, r = -0.208) (Table 3, Fig 2). Linear regression analysis of SPARC-positive percentage of individual cases in MSC showed significant correlation with MVD in these human colon cancer specimens (P < 0.05, r = -0.578) (Table 3, Fig 3). Figure 2 Correlation analysis of SPARC expression in MSC and VEGF expression in colon cancer. Figure 3 Linear regression analysis of the percentage of SPARC stained in MSC with MVD. Survival analysis Kaplan-Meier analysis and the log-rank test were used to evaluate the effects of the SPARC and VEGF expression on survival.