2002). Subjects with other axis I diagnosis, active suicidality, unstable medical conditions, current

or past history of thyroid disease or abnormal thyroid function tests, or a positive urine toxicology screen were excluded. Assessments Depressive symptoms were rated over eight visits (Screening, day 3, weeks 1, 2, 3, 4, 5, and 6) using the following rating SKI-606 chemical structure scales: Montgomery and Asberg Depression Rating Scale (MADRS) (Montgomery and Asberg 1979), Beck Depression Inventory (BDI) (Beck et al. 1961), Clinical Global Impression – Severity of illness (CGI-S) (Guy 1976), Scale for Suicidal Ideation (SSI) (Beck et al. 1979). Medications All subjects received open label citalopram (20 mg) for 6 weeks Inhibitors,research,lifescience,medical and were randomized in a blinded fashion to receive additionally triiodothyronine (T3) 25 μg BID (n = 7), pindolol 5 mg BID (n = 8), or placebo (n = 8) at the start of citalopram treatment. Thyroid function tests Serum TFTs were checked at baseline and at the end visit. TSH and total triiodothyronine Inhibitors,research,lifescience,medical (TT3) were assessed by immunoassay (ROCHE Elecsys 170 Analyzers, Roche Diagnostics, Indianapolis, IN), free triiodothyronine (FT3), and free thyroxine (FT4) by Enzyme Immunoassay Assay Diagnostic System Laboratory (EIA-DSL). Statistical analysis Primary outcome measure was the time to 50% reduction in baseline MADRS scores. Collected data were screened for distributional properties

Inhibitors,research,lifescience,medical and determined to be appropriate for parametric analysis. Simple correlation analysis and proportional hazard regression (Cox Model) and accelerated failure time survival regression analysis were used

to predict time to response (i.e. 50% improvement in MADRS scores) and remission (MADRS score ≤ 7) with baseline Inhibitors,research,lifescience,medical and delta TSH, FT4, FT3, and TT3 as independent variables. Analysis was done using SPSS software (SPSS IBM, Armonk, New York). Results Demographics and baseline depression scores Of the randomized 23 subjects, 19 completed the study. Two subjects Inhibitors,research,lifescience,medical in the placebo group dropped out (one due to worsening of depression, and the other one due to excessive use of lorazepam) and two subjects missed follow-up. The mean age of the cohort was 38.34 (±12.6) years and the mean length of the index episode was 8.9 (±5.9) months with an age of onset of 32.9 (±13.5) years. All patients were required to not be receiving antidepressants for at least one month prior to starting. All, but five, patients were antidepressant naive at the time of the through study. The mean baseline MADRS score was 29.7 (±5.85), BDI score was 23.4 (±7.3), and a mean CGI severity score was 4.1 (±0.3). Six patients met DSM-IV criteria of life comorbid generalized anxiety disorder, four with posttraumatic stress disorder, and one patient with social phobia. All comorbid conditions were clinically stable and none of the patients receives treatment or therapy for comorbid conditions during the study.