2). Radioimmunity was applied to measure the plasma motilin level Selleckchem IDH inhibitor before and after ghrelin administration. 3). The c-Fos activation on the CNS and ENS through

intravenous injection of ghrelin was studied by the immunohistochemistry. Results: 1). Ghrelin showed an excitatory effect on gastrointestinal IMC. This effect was inhibited by atropine, L-arginine, ondansetron or (D-Lys3)GHRP-6, but not by propranolol and phentolamine. 2). The plasma motilin level in different phases of IMC of the normal rats had cyclical fluctuation with the lowest level in phase I, and the highest level in phase III. After injection of ghrelin, the cyclical fluctuation was not affected, and the motilin X-396 level had little difference in each corresponding phase compared with that before ghrelin administration. 3). In the CNS, the c-Fos expression of several nuclei such as the arcuate nucleus, paraventricular nucleus, and so on, was increased by injection of ghrelin. And the c-Fos expression of the duodenum, jejunum, and proximate colon was also activated by ghrelin. Conclusion: Ghrelin appears to play an important role in regulating of intestinal motility. Its excitatory effect relies on the cholinergic pathway

and is closely related to the NOS-NO or 5-HT pathway. Ghrelin receptor GHS-R regulates its activity. The excitatory effects of ghrelin on the intestinal IMC don’t have relationship with plasma motilin level. Intravenous administration of ghrelin could regulate the intestinal motility through the ENS or CNS. Key Word(s): 1. 上海皓元 ghrelin; 2. IMC; 3. motilion; 4. c-Fos; Presenting Author: JUANIGNACIO TELLECHEA Additional Authors: FRANCOPABLO BELLAVITE, NICOLAS SALIM, CAROLINA BOLINO, HORACIO VAZQUEZ, GUIDO IANTORNO Corresponding Author: JUANIGNACIO TELLECHEA, FRANCOPABLO BELLAVITE Affiliations: None Objective: The World Health

Organization (WHO) estimates that Chagas Disease (Ch D) affects 16 to18 million people worldwide. It is considered endemic in America. Argentina has 2,5 million infected people and 10 million people are exposed to infection. The cardiac affection is the most frequent and has been studied extensively. Gastrointestinal compromise is present in less than 11% and most affected organs are esophagus and colon. The impact of colon affection in our country is unknown. Objectives: 1. Estimate the prevalence of Ch D in chronic constipation (CC) patients. 2. Characterize the sample according to systemic compromise, place of origin, radiologic findings and presence or absence of Rectoanal Inhibitory Reflex (RAIR). Methods: We reviewed medical records of adult patients ≥18 years old who were referred for chronic constipation. Patients with positive serology for Ch D (ELISA, IFI) were included; other reasons for chronic constipation were exclusion criteria.