1%, 92.3% and 94.7% respectively. IgM/IgG ratio of serous inflammatory cells was higher in PBC group than in AIH group. In liver tissues, AIH cases showed predominant IgG immunostaining in portal area (66.7 %). IgM + /IgG+ ≧1 took up 100% patients in PBC group and 84.6% patients in OS group. But half of PBC cases showed slightly difference between IgG and IgM. Conclusion: IgM/IgG ratio of inflammatory cells in serum and liver biopsy tissues can be a valuable parameter for differentiating PBC from AIH. Key Word(s): 1.

autoimmune hepatitis; 2. overlap syndrome; 3. IgG; 4. IgM; Presenting Author: KITTIYOD POOVORAWAN Additional Authors: PALITTIYA SINTUSEK, NIPAPORN SIRIPON, SOMBAT TREEPRASERTSUK, PISIT TANGKIJVANICH, YONG POOVORAWAN, PIYAWAT KOMOLMIT Corresponding Author: KITTIYOD POOVORAWAN Affiliations: Faculty of Medicine, Chulalongkorn University Objective: Spleen stiffness has been found correlated with certain degrees of portal hypertension BAY 80-6946 (PHT). This study has been aimed at comparing and validating spleen stiffness measurement with and without US guidance and its correlation with clinical significance. Methods: Thirty healthy volunteers were recruited. Demographic Small molecule library data including weight, height and

BMI were collected. Patients were subjected to crossover measurement by fibroscan of the area between 2–4 cm below the mid axillary line at the intercostal space between the 8th and 9th rib without US guidance (Figure 1) in comparison with the ultrasound guided method. Spleen stiffness level, IQR and success rate were recorded and crossover analysis was performed. This method was applied on patients with the clinical significance of PHT. Clinical outcomes (degrees 上海皓元 of esophageal varices, spleen size and platelet count) and degree of spleen stiffness were compared.

Results: Healthy males (10) and females (20) at a median age of 30 years (ranging from 22–54 years) were recruited. Median BMI was 22.1. (ranging from 16.8–35.1) Mean spleen stiffness levels were 18.3 ± 13.4 kPa and 18.4 ± 11.4 kPa (p = 0.57), mean success rates were 46.9 ± 27% and 42.4 ± 27.7% (p = 0.86), mean IQR were 4.4 ± 4.2 and 5.9 ± 6.5 (p = 0.1) applying the non US guided technique and the US guided technique, respectively. No statistically significant difference in results between these two techniques was detectable. Spleen stiffness levels were significantly correlated (r = 0.75, p < 0.01). The mean operative time was less with the non-US guided technique (5.5 min vs. 9.1 min, p = 0.05). This method was applied on 38 patients with biliary atresia and 11 patients with extra-hepatic PHT. Higher success rate (89.9 ± 18%) and less operative time (3.3 ± 3.2 minutes) were observed in these groups of patients. Degree of spleen stiffness and clinical outcomes (degrees of esophageal varices and platelet count) were significantly correlated (r = 0.57 and r = −0.64; p < 0.01, respectively).