Mean hemolysis for genomospecies A isolates was find more greater than for isolates belonging to genomospecies B (64.0 ± 4.9% and 45.2 ± 5.1%, respectively; P = 0.027). Mean hemolysis did not differ between isolates from healthy and diarrheic individuals (55.9 ± 8.2% versus 52.0 ± 5.2%, respectively; P = 0.68), nor between isolates assigned to AFLP clusters 1 and 2 (63.9 ± 6.0% versus 47.5 ± 5.0%, EPZ5676 molecular weight respectively; P = 0.06). There was an inverse correlation between hemolysis and invasion Rabusertib (R2 = 0.74; P < 0.0001) and between hemolysis and adherence (R2 = 0.43; P < 0.011). None of the C. concisus isolates caused significant epithelial cytotoxicity, whereas Campylobacter jejuni 81-176 and H2O2 induced cytotoxicity in agreement with previous observations

[25] (Table 3). Table 3 Hemolysis, DNA fragmentation, cytotoxicity, and metabolic activity of Campylobacter concisus isolatesa. Isolate AFLP Cluster Hemolysisb (%) DNA fragmentationc (A370 nm) Cytotoxicityc (%) Metabolic activity c (% control) CHRB2004 1 60.2 ± 14.4 1.84 ± 0.17d 1.23 ± 0.21 139.4 ± 7.4 CHRB3287 1 45.6 ± 16.9 1.83 ± 0.13d 1.48 ± 0.16 146.8 ± 9.2 CHRB2011 1 60.5 ± 9.8 1.63 ±.0.05d 0.88 ± 0.22 151.9 ± 7.5 CHRB3290 1 81.1 ± 4.5 1.91 ± 0.14d 0.94 ± 0.19 155.7 ± 2.3 CHRB1609 1 72.3 ± 9.4 1.37 ± 0.18 1.11 ± 0.34 144.5 ± 4.4 CHRB1794 2 70.9 ± 10.1 1.32 ± 0.19 1.42 ± 0.15 137.9 ± 2.9 CHRB6 2 41.2 ± 11.6 1.12 ± 0.26 1.43 ± 0.18 105.1 ± 26.2e CHRB1569 2 47.0 ± 12.0 1.38 ± 0.17 1.29 ± 0.26 139.2 ± 7.0 CHRB2691 2 62.1 ± 14.3 1.62 ± 0.07d 1.89 ± 0.15 133.5 ± 10.3 CHRB2370 2 44.9 ± 12.0 1.69 ±

PIK3C2G 0.14d 1.46 ± 0.08 142.8 ± 6.5 CHRB2050 2 64.3 ± 15.4 1.41 ± 0.07 0.97 ± 0.15 131.0 ± 7.1 CHRB563 2 34.6 ± 13.9 1.55 ± 0.23d 1.25 ± 0.20 138.0 ± 10.2 CHRB3152 2 30.7 ± 15.4 1.89 ± 0.16d 1.28 ± 0.15 141.0 ± 6.0 CHRB3235 2 32.1 ± 18.6 1.69 ± 0.12d 1.14 ± 0.16 143.2 ± 6.3 LMG7788 1 61.5 ± 10.8 1.54 ± 0.08d 0.71 ± 0.10 140.8 ± 5.2 C. jejuni 81-176 — 75.6 ± 3.7 1.68 ± 0.25d 4.53 ± 0.31d 143.7 ± 5.7 Broth control — 0.44 ± 0.14 0.69 ± 0.12 0.96 ± 0.34 100 H2O2 — – 1.38 ± 0.22 6.15 ± 1.66d 259.5 ± 13.5 Camptothecin — – 2.23 ± 0.40d 1.39 ± 0.28 177.5 ± 9.2 a Data are means ± SEM, n = 3. b Percent total hemolysis of sheep erythrocytes for 1/8 dilution of Campylobacter inoculum. c Assays conducted using T84 monolayers d P < 0.05 relative to the broth control treatment. e Sloughing of epithelial cells noted in two of three repetitions.