The strain characteristics are reported in Table 1. Out of the 22 Dactolisib price strains tested, six strains were isolated from patients with GC, three strains from cases of DU and the others from patients with CGO. Sixteen strains possessed the cagA gene; strain 328 Km was a cagA-negative isogenic mutant of the wild LOXO-101 molecular weight cagA-positive isolate 328 (Table 1). Table 1 Characteristics of H. pylori strains tested Parameter Helicobacter pyloristrains   CCUG 17874 G50 G21 4Kb DiSim 10 K 328 328 Km* M/C-R1 M/C-R2 M/C-R3 Ap-R 3Cb Marit G27 17C7 Ba142 12A3 8C8 G104 Ver1 Ver2 Presence of cagA gene + – - + + + + – + – + + + + + + – + + – + + Pathology of patients CGO CGO CGO GC DU GC CGO

CGO CGO CGO CGO DU GC CGO DU GC CGO GC GC CGO CGO CGO Primary strain Yes Yes Yes Yes Yes Yes Yes Yes No No No No Yes No Yes Yes Yes Yes Yes Yes No No * This is an isogenic cagA negative mutant of the wild strain 328. CGO: chronic gastritis only; DU: duodenal ulcer; GC: gastric carcinoma. Determination of the chemosusceptibility of H. pylori strains to polysorbate 80 and antibiotics The results of the chemosusceptibility tests are expressed in μg/mL and are reported in Table 2 as mean and standard deviation in parentheses. MBCs

of polysorbate 80 ranged from 2.6 (1.1) to 32 (0) (Table 2); the MBC50 (the concentration at which ≥50% of strains were killed) was 16 (0). All strains were susceptible to amoxicillin (< 1.0 μg/ml) and MBCs ranged from 0.002 (0) to 0.6 (0.1); the MBC50 Selleck Combretastatin A4 was 0.03 (0) (Table 2). Five secondary isolates (23.9%), were resistant to

clarithromycin (> 1.0 μg/ml) (Table 2). Two strains presented a high level of resistance with MBC of 320 (0) and 2500 (0), while MBC of the other strains were 32 (0) for two strains and 64 (0) (Table 2). MBCs for the susceptible strains ranged from 0.01 (0) to 0.5 (0) (Table 2) and the MBC50 was Methisazone 0.08 (0). Eight strains (36.3%, four strains were secondary) were resistant to metronidazole (>4 μg/ml) (Table 2); MBCs for resistant strains were 20.8 (7.2), 21.3 (9.2), 26.6 (9.2), 32 (0), 64 (0), 128 (0) for two strains and 170.6 (73.9) (Table 2). All strains, excepted one primary strain, were susceptible to levofloxacin (<2 μg/ml) (Table 2); MBCs ranged from 0.12 (0) to 0.5 (0) and the MBC50 was 0.25 (0) (Table 2). Finally, one primary and one secondary strains (9.0%) were resistant to tetracycline with MBC of 4 (0) and 6.6 (2.3); one strain was also resistant to metronidazole and clarithromycin, the other strain to metronidazole only. MBCs of tetracycline for the susceptible strains (< 4 μg/ml) ranged from 0.03 (0) to 2 (0) and the MBC50 was 0.25 (0). Table 2 MBCs of polysorbate 80, antibiotics and association of polysorbate 80 and antibiotics to the H.