0002) and in addition rhizomes (P = 0.0386) at the dry sites. Comparisons between the two mTOR activator species showed that roots were the only organs with significantly contrasting preferences for the habitat type (root-flooded: P = 0.0213; root-dry: P = 0.00004) (Figure 3, capital letters). Figure 3 Habitat preferences of Microdochium spp. on Lake Constance reeds. Summary of nested-PCR assays on 251 DNA preparations from tissue samples of P. australis. Detection frequency for each target shows the percentage of samples producing a SAHA HDAC band after the second step of the nested-PCR. Results from all seasons were pooled. Small letters compare variation between the two habitat types when analyzing each target species and each host organ separately (binomial
test with P <0.05). Capital letters compare variation between the two species when analyzing each CYC202 ic50 host organ and each habitat separately (binomial test with P <0.05). S/s, variation is significant; non-significant variation is not indicated. Underlined letters indicate that the variation remains significant after Bonferroni correction. Carbon utilization patterns of Microdochium spp To determine whether resource partitioning, as a biotic attribute, may have contributed to these findings the potential of Microdochium spp. to utilize 95 different carbon sources was tested in vitro. The EcoSim Niche Overlap module was used to evaluate the overall similarity in
carbon usage. The niche overlap index in the experimentally obtained data set was 0.9733, whereas the mean of the simulated matrices was 0.7127, using default parameters for calculation (RA3 model). This difference was statistically significant (P < 0.05), and thus indicated that the carbon usage of the two species was overall more similar than expected by chance. The application of alternative parameters for the calculation (i.e. the RA1, RA2, and RA4 models) led to the same conclusion. In addition,
intra-species comparison of different strains belonging to the same species showed that within each of the two species there were significantly more resource overlaps than expected by chance (data not shown). Although the carbon utilization capabilities of the two species Proteases inhibitor were similar, specific differences existed, which were statistically assessed using t-tests. Significant differences between the two species (P < 0.05) were observed for 21 substrates (22.1%) (Additional file 3). In addition, the application of the Dunnett test rendered essentially the same results (not shown). M. bolleyi grew significantly better than M. phragmitis on 10 of the 95 carbon sources tested (Additional file 3). Conversely, M. phragmitis grew significantly better than M. bolleyi on 11 carbon sources (Additional file 3). Temperature ranges for growth of Microdochium spp The potential effect of temperature, as an abiotic attribute, was tested to determine if it could distinguish these fungi and explain their observed distributions in field samples.