The activation of hepatic macrophages leads to an exacerbation of hepatic inflammation. PRIMARY SCLEROSING CHOLANGITIS (PSC) is a chronic cholestatic liver disease characterized by inflammation, obliteration and fibrosis of the intrahepatic and/or extrahepatic biliary ducts.[32] Although the etiology of PSC remains unknown, gut microbiota are considered to play an important role in the pathogenesis of PSC. Sumitran-Holgersson et al.[33, 34] reported that approximately 60% of PSC patients have serum antibodies to BEC (anti-BEC), and stimulation of BEC with the immunoglobulin

(Ig)G of anti-BEC+ PSC patients induces the expression of TLR4, whereas unstimulated or normal IgG-stimulated BEC do not express TLR4. TLR4-expressing BEC produce high levels of IL-1β, IL-8 and Dabrafenib datasheet IFN-γ when stimulated with LPS. Mueller et al.[35] reported that BEC from end-stage PSC liver show marked expression of TLR4, increased activation of the myeloid differentiation protein 88/IL-1 receptor-associated kinase signaling cascade, and a loss of immune tolerance to endotoxin after repeated endotoxin exposure. However, the expression of TLR4 in BEC from the early-stage PSC liver is similar to that of healthy liver.

Increased expression of TLR4 and a loss of immune tolerance to endotoxin in BEC may coordinate autoimmunity in the progression of PSC. Approximately 1–3% of patients with ulcerative colitis (UC) had concurrent PSC,[36-38] and approximately 68–75% of PSC patients had UC.[39-41] UC patients with PSC more frequently have total colonic involvement than UC patients without PSC FK228 research buy (68–85% vs 44–45%).[36, 42] In UC patients, the extent of disease is positively correlated with plasma concentrations of endotoxin.[43] Thus, endotoxin concentrations in the portal vein are expected to be higher in UC patients with PSC than in UC patients without PSC. Furthermore, in the bile of PSC patients,

enteric bacteria such as Escherichia coli are frequently detected.[44] Thus, in PSC patients, the liver constantly confronts abundant gut bacterial antigens such as endotoxin, and reinforced confrontation with these antigens is considered to be among the causes MCE公司 of PSC. Serum perinuclear antineutrophil cytoplasmic antibodies (pANCA), which are frequently seen in patients with UC, have been detected in approximately 80% of PSC patients.[45, 46] By indirect immunofluorescence study, pANCA in PSC show a heterogeneous rim-like staining in the nuclear periphery (atypical pANCA),[47] unlike classical pANCA, which show peripheral rim-like staining without intranuclear staining in patients with systemic vasculitis. Recently, the autoantigen of this atypical pANCA has been reported to be β-tubulin isotype 5.[48] Furthermore, this atypical pANCA cross-reacts with FtsZ, which is present in almost all bacteria of the gut microbiota.